Humanized Monoclonal Antibodies as a Synthetic Serum Alternative
As a highly reliable and readily available alternative to human serum, SERION Immunologics offers humanized monoclonal antibodies.
The performance of these monoclonal antibodies, in comparison to human disease state sera, has been tested extensively by methods such as titration, dilution in various matrices, reactivity at different temperatures and their behavior after addition of rheumatoid factor absorbent (Rf-stripper). The best performing clones - selected as sales products - show a behavior nearly identical to the corresponding human sera. Therefore, they can be optimally and alternatively used for preparation of calibrators, controls and standards.
The monoclonal antibodies are offered in two different matrices. The original product line is provided as a cell culture supernatant packaged in 1 mL up to 1 L quantities. The new generation of antibodies is available in an IgG depleted and delipidized human serum matrix with 100 µg up to 25 mg packaging sizes. Production lots are usually offered with a concentration between 100 µg/mL and 150 µg/mL.
Test samples can be offered free of charge.
Features and benefits:
- High affinity
- High specificity
- Industrial batch sizes
- Constant availability
- Constant quality and performance
SERION antibodies are produced in transgenic mice by replacing the mouse sequence of the heavy chain constant region (IgM, IgG or IgA loci) with the corresponding human sequence. After immunization with the antigen of interest, generated antibody clones are cultivated by standard hybridoma techniques. These consist of the human constant region of the heavy chain, mouse variable region of the heavy chain and mouse light chain. The human constant region of the heavy chain can be directly recognized by the anti-human conjugate, which is used in numerous in vitro diagnostic assays.
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