Bordetella pertussis is a Gram-negative, encapsulated coccobacillus of the genus Bordetella, and the causative agent of pertussis or whooping cough. The different antigens from B. pertussis – Pertussis toxin (PT), Filamentous hemagglutinin (FHA) and the whole-cell antigen – are produced by cultivation of B. pertussis, Strain Tohama I. PT and FHA are highly purified (purity in Coomassie SDS-PAGE ≥ 95 %), cross reactivity is ruled out by Western Blot and both antigens are suitable for the detection of IgG and IgA antibodies against B. pertussis. The whole-cell antigen is harvested from broth culture and suitable for the detection of IgM antibodies.
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