The genus Chlamydia includes, among others, the human and animal pathogenic species C. pneumoniae, C. trachomatis and C. abortus. For the production of the native antigens, strains C. pneumoniae TWAR strain TW-183 and C. abortus strain B-577 are cultivated in cell culture (Vero or BGM cells) and the elementary and reticular bodies are prepared by ultra-centrifugation. In case of C. pneumoniae a further purified native antigen is available – the COMC premium antigen. This antigen is prepared by detergent extraction of the reticular bodies to prepare chlamydial outer membrane complexes (COMCs). This premium antigen is highly recommended for the detection of IgG, IgM and IgA antibodies against C. pneumoniae. Additionally, a recombinant C. trachomatis MOMP antigen is available which is produced in E. coli, purified by chromatography in the presence of urea. Purity of ≥ 95 % is confirmed via SDS-PAGE (Coomassie staining) and the antigen is suitable for the detection of IgG, IgM and IgA antibodies against C. trachomatis.
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