Leptospira is a genus of spirochetes bacteria, including a small number of pathogenic and saprophytic species. Leptospira biflexa is a non-pathogenic organism, which can be used as a prototype antigen for the development and manufacturing of diagnostics reagents like ELISA. For the production of the native antigen Leptospira biflexa, strain Patoc 1 (serovar Patoc) is cultivated in broth culture and after harvest extracted by ethanol. The antigen is suitable for the detection of IgM and IgG antibodies against Leptospira ssp.
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