Mycoplasma pneumoniae is a small bacterium lacking the peptidoglycan cell-wall and belongs to the Mollicutes class. It is a human pathogen and causes the disease mycoplasma pneumonia, a form of an atypical bacterial pneumonia which is sometimes difficult to treat by antibiotics. For the manufacturing of the native antigen Mycoplasma pneumoniae (strain FH) is cultivated in broth culture and the organisms are detergent extracted to produce the P1-adhesin enriched antigen. The antigen is suitable for the detection of IgG and IgM antibodies against M. pneumoniae. Additionally, also a recombinant P1-adhesin protein is available. This antigen is produced in E.coli, purified by affinity chromatography in the presence of urea and suitable for the detection of IgG antibodies.
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